Anticorps Monoclonal anti-CD82

CD82 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

CloneNo.

1E7A4

N° de cat : 66803-1-PBS

Synonymes

CD82, KAI1, 1E7A4, CD 82, CD82,KAI1, IA4

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Galerie de données de validation

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  WB analysis using 66803-1-Ig (same clone as 66803-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66803-1-Ig (CD82 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66803-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66803-1-Ig (same clone as 66803-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66803-1-Ig (CD82 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66803-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66803-1-Ig (same clone as 66803-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66803-1-Ig (CD82 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66803-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66803-1-Ig (same clone as 66803-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66803-1-Ig (CD82 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66803-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 66803-1-Ig (same clone as 66803-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CD82 antibody (66803-1-Ig, Clone: 1E7A4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66803-1-PBS in a different storage buffer formulation.

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  IF Staining of human ovary tumor using 66803-1-Ig (same clone as 66803-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human ovary tumor tissue using CD82 antibody (66803-1-Ig, Clone: 1E7A4 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66803-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66803-1-PBS cible CD82 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CD82 Protéine recombinante Ag17513
• Nom complet: CD82 molecule
• Masse moléculaire calculée: 30 kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: BC000726
• Symbole du gène: CD82
• Identification du gène (NCBI): 3732
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD82 is a membrane glycoprotein and belongs to the tetraspanin superfamily, many of which are implicated in the regulation of cell motility, morphology, fusion, signaling, fertilization, and differentiation. CD82 was originally identified as a suppressor of metastasis located on human chromosome 11p11.2 in prostate carcinoma. The majority of evidence indicates that CD82 expression is downregulated or abolished in a variety of malignant tumors. CD82 is present at high levels in human monocyte and macrophage lineages and in various epithelial cells in the prostate, lung, pancreas and many other tissues. In epithelial cells, CD82 is implicated in diverse biological processes such as cell adhesion, migration, apoptosis and morphogenesis.

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