Anticorps Recombinant de lapin anti-CD82

CD82 Recombinant Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

241765G7

N° de cat : 84617-7-RR

Synonymes

CD82, KAI1, Metastasis suppressor Kangai-1, IA4, CD82,KAI1, CD 82

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Galerie de données de validation

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  WB analysis of Jurkat using 84617-7-RR

  Jurkat cells were subjected to SDS PAGE followed by western blot with 84617-7-RR (CD82 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 84617-7-RR

  Raji cells were subjected to SDS PAGE followed by western blot with 84617-7-RR (CD82 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-87 MG using 84617-7-RR

  U-87 MG cells were subjected to SDS PAGE followed by western blot with 84617-7-RR (CD82 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary cancer using 84617-7-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84617-7-RR (CD82 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary cancer using 84617-7-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84617-7-RR (CD82 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 84617-7-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84617-7-RR (CD82 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 84617-7-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84617-7-RR (CD82 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  Affinity and Kinetic Characterization of 84617-7-RR

  Biolayer interferometry (BLl) kinetic assays of 84617-7-RR against Human CD82 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules MG U-87, cellules Raji
• Résultats positifs en IHC: tissu d'amygdalite humain, human ovary cancer tissue; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84617-7-RR cible CD82 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: CD82 molecule
• Masse moléculaire calculée: 30 kDa
• Poids moléculaire observé: 35-45 kDa
• Numéro d’acquisition GenBank: NM_002231.4
• Symbole du gène: CD82
• Identification du gène (NCBI): 3732
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD82 is a membrane glycoprotein and belongs to the tetraspanin superfamily, many of which are implicated in the regulation of cell motility, morphology, fusion, signaling, fertilization, and differentiation. CD82 was originally identified as a suppressor of metastasis located on human chromosome 11p11.2 in prostate carcinoma. The majority of evidence indicates that CD82 expression is downregulated or abolished in a variety of malignant tumors. CD82 is present at high levels in human monocyte and macrophage lineages and in various epithelial cells in the prostate, lung, pancreas and many other tissues. In epithelial cells, CD82 is implicated in diverse biological processes such as cell adhesion, migration, apoptosis and morphogenesis.

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