Anticorps Recombinant de lapin anti-CD83

CD83 Recombinant Antibody for IF/ICC, FC

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, primates non humains

Applications

IF/ICC, FC

Conjugaison

Non conjugué

CloneNo.

HB15e

N° de cat : 65581-1-MR

Synonymes

B cell activation protein, B-cell activation protein, BL11, CD83 antigen, CD83 molecule

---

Galerie de données de validation

• 

  IF Staining of Raji using 65581-1-MR

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD83 antibody (65581-1-MR, Clone: HB15e ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

• 

  FC experiment of human monocyte-derived mature dendritic cells using 65581-1-MR

  1x10^6 human monocyte-derived mature dendritic cells were surface stained with 0.25 ug Anti-Human CD83 Mouse IgG2a Recombinant Antibody (65581-1-MR, Clone: HB15e) (red) or 0.25 ug Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

---

Applications testées

• Résultats positifs en IF/ICC: cellules Raji,
• Résultats positifs en cytométrie: Human monocyte-derived mature dendritic cells

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

65581-1-MR cible CD83 dans les applications de IF/ICC, FC et montre une réactivité avec des échantillons Humain, primates non humains

• Réactivité: Humain, primates non humains
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Cellules Cos humaines transfectées par CD83
• Nom complet: CD83 molecule
• Masse moléculaire calculée: 205 aa, 23 kDa
• Numéro d’acquisition GenBank: BC030830
• Symbole du gène: CD83
• Identification du gène (NCBI): 9308
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Stable for one year after shipment.

Informations générales

CD83 is a member of the immunoglobulin (Ig) superfamily, consisting of an extracellular V-type Ig-like domain, a transmembrane domain, and a cytoplasmic tail. CD83 is one of the most prominent markers for fully matured dendritic cells (DCs) (PMID: 17334966). It is also found on the surface of other activated hematopoietic cells, including lymphocytes, monocytes, macrophages, and neutrophils (PMID: 31231400; 17334966). CD83 regulates the maturation, activation, and homeostasis of numerous immune cells (PMID: 31231400; 32362900). CD83 can also expressed as a soluble form. Soluble CD83 can bind to DCs and inhibit their maturation (PMID: 17334966; 12403928).

{{ptg:RelatedPrimaryAntibodies}}