Anticorps Recombinant de lapin anti-CD83

CD83 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

FC

Conjugaison

Non conjugué

CloneNo.

241002B7

N° de cat : 98119-1-RR

Synonymes

241002B7, CD83 antigen, mCD83

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Galerie de données de validation

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  FC experiment of mouse splenocytes using 98119-1-RR

  1x10^6 untreated or LPS-treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD83 Rabbit Recombinant Antibody (98119-1-RR, Clone: 241002B7) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using 98119-1-RR

  1x10^6 LPS-treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD83 Rabbit Recombinant Antibody (98119-1-RR, Clone: 241002B7) (red) or 0.25 ug Isotype Control (blue), and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD83 Rabbit Recombinant Antibody (98119-1-RR, Clone: 241002B7) (black, dashed), and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98119-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98119-1-RR against Mouse CD83 were performed. The affinity constant is 0.199 nM.

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Applications testées

• Résultats positifs en cytométrie: LPS treated mouse splenocytes,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98119-1-RR cible CD83 dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD83 Protéine recombinante Eg1387
• Nom complet: CD83 antigen
• Masse moléculaire calculée: 21 kDa
• Numéro d’acquisition GenBank: NM_009856.3
• Symbole du gène: Cd83
• Identification du gène (NCBI): 12522
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

CD83 is a member of the immunoglobulin (Ig) superfamily, consisting of an extracellular V-type Ig-like domain, a transmembrane domain, and a cytoplasmic tail. CD83 is one of the most prominent markers for fully matured dendritic cells (DCs) (PMID: 17334966). It is also found on the surface of other activated hematopoietic cells, including lymphocytes, monocytes, macrophages, and neutrophils (PMID: 31231400; 17334966). CD83 regulates the maturation, activation, and homeostasis of numerous immune cells (PMID: 31231400; 32362900). CD83 can also expressed as a soluble form. Soluble CD83 can bind to DCs and inhibit their maturation (PMID: 17334966; 12403928).

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