Anticorps Monoclonal anti-CD86

CD86 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain et plus (1)

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(2)

CloneNo.

1D6H7

N° de cat : 68674-2-Ig

Synonymes

Activation B7 2 antigen, B7 2, B70, BU63, CD28LG2, CD86, CD86 molecule, CTLA 4 counter receptor B7.2, FUN 1, LAB72

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Galerie de données de validation

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  WB analysis using 68674-2-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68674-2-Ig (CD86 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68674-2-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68674-2-Ig (CD86 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules Raji, cellules Daudi, cellules Ramos

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

68674-2-Ig cible CD86 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: CD86 molecule
• Masse moléculaire calculée: 329 aa, 38 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: NM_175862
• Symbole du gène: CD86
• Identification du gène (NCBI): 942
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD86 (also known as B7.2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response.

Publications

Species	Application	Title
human	WB	Aesthetic Plast Surg; Complications of Polyacrylamide Hydrogel Facial Injection: Clinical Studies and Literature Review; Authors - Haifeng Yao
mouse	WB	Pharmaceutics; Multi-Omics Analysis of the Immune Effect of the Engineered Exosome Drug Delivery System in Inducing Macrophage Apoptosis; Authors - Wei Xiang

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