Anticorps Recombinant de lapin anti-CD86

CD86 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

240429G12

N° de cat : 83523-4-PBS

Synonymes

240429G12, Activation B7 2 antigen, Activation B7-2 antigen, B7 2, B70

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Galerie de données de validation

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  WB analysis using 83523-4-RR (same clone as 83523-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83523-4-RR (CD86 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83523-4-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 83523-4-RR (same clone as 83523-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83523-4-RR (CD86 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83523-4-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 83523-4-RR (same clone as 83523-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83523-4-RR (CD86 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83523-4-PBS in a different storage buffer formulation.

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  IF Staining of Raji using 83523-4-RR (same clone as 83523-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD86 antibody (83523-4-RR, Clone: 240429G12 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83523-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83523-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83523-4-RR against Human CD86 were performed. The affinity constant is 0.766 nM.

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Informations sur le produit

83523-4-PBS cible CD86 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD86 Protéine recombinante Eg0966
• Nom complet: CD86 molecule
• Masse moléculaire calculée: 329 aa, 38 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: NM_175862
• Symbole du gène: CD86
• Identification du gène (NCBI): 942
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD86 (also known as B7.2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response.

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