Anticorps Recombinant de lapin anti-CD86

CD86 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

FC

Conjugaison

Non conjugué

CloneNo.

230476B8

N° de cat : 98025-1-PBS

Synonymes

B7-2, B70, B7.2, B7 2, B7

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Galerie de données de validation

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  FC experiment of mouse splenocytes using 98025-1-RR (same clone as 98025-1-PBS)

  1x10^6 unstimulated or LPS stimulated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD86 Rabbit Recombinant Antibody (98025-1-RR, Clone:230476B8) and PE-Conjugated Goat Anti-Rabbit IgG (H+L). Cells were not fixed. This data was developed using the same antibody clone with 98025-1-PBS in a different storage buffer formulation.

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  FC experiment of mouse splenocytes using 98025-1-RR (same clone as 98025-1-PBS)

  1x10^6 unstimulated (black) or LPS stimulated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD86 Rabbit Recombinant Antibody (98025-1-RR, Clone:230476B8) and PE-Conjugated Goat Anti-Rabbit IgG (H+L) (red), or 0.25 ug Isotype Control (blue). Cells were not fixed. This data was developed using the same antibody clone with 98025-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98025-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98025-1-RR against Mouse CD86 were performed. The affinity constant is 25.4 nM.

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Informations sur le produit

98025-1-PBS cible CD86 dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: CD86 antigen
• Masse moléculaire calculée: 35 kDa
• Numéro d’acquisition GenBank: NM_019388
• Symbole du gène: Cd86
• Identification du gène (NCBI): 12524
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C.

Informations générales

CD86 (also known as B7-2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and CTLA-4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with CTLA-4 negatively regulates T-cell activation and diminishes the immune response. (PMID: 7513726; 1847722; 11029388; 27591335)

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