Anticorps Recombinant de lapin anti-CD86

CD86 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

CloneNo.

240429C6

N° de cat : 98043-1-RR

Synonymes

BU63, B70, B7 2, Activation B7-2 antigen, Activation B7 2 antigen

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Galerie de données de validation

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  FC experiment of human PBMCs using 98043-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD86 Rabbit Recombinant Antibody (98043-1-RR, Clone: 240429C6) or 0.25 ug Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cells were then stained with APC Anti-Human CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using 98043-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD86 Rabbit Recombinant Antibody (98043-1-RR, Clone: 240429C6) (red) or 0.25 ug Isotype Control (blue) and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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  Affinity and Kinetic Characterization of 98043-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98043-1-RR against Human CD86 were performed. The affinity constant is 0.23 nM.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98043-1-RR cible CD86 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: CD86 molecule
• Masse moléculaire calculée: 329 aa, 38 kDa
• Numéro d’acquisition GenBank: NM_175862
• Symbole du gène: CD86
• Identification du gène (NCBI): 942
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Stable for one year after shipment.

Informations générales

CD86 (also known as B7-2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and CTLA-4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with CTLA-4 negatively regulates T-cell activation and diminishes the immune response. (PMID: 7513726; 1847722; 11029388; 27591335)

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