Anticorps Monoclonal anti-CD86

CD86 Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

Humain

Applications

FC

Conjugaison

APC Fluorescent Dye

Publications

1

CloneNo.

BU63

N° de cat : APC-65165

Synonymes

Activation B7 2 antigen, B7 2, B70, BU63, CD28LG2, CD86, CD86 molecule, CTLA 4 counter receptor B7.2, FUN 1, LAB72

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Galerie de données de validation

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  FC experiment of human PBMCs using APC-65165

  1X10^6 human PBMCs were surface co-stained with FITC Anti-Human CD14 and 5 ul APC Anti-Human CD86 (APC-65165, Clone:BU63) or unstained. Cells were not fixed. Monocytes were gated.

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  FC experiment of human PBMCs using APC-65165

  1X10^6 human PBMCs were surface stained with 5 ul APC Anti-Human CD86 (APC-65165, Clone:BU63) or unstained. Cells were not fixed. Monocytes were gated.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines,

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Applications publiées

• FC: See 1 publications below

Informations sur le produit

APC-65165 cible CD86 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Lignée cellulaire B-lymphoblastoïde ARH 77
• Nom complet: CD86 molecule
• Masse moléculaire calculée: 329 aa, 38 kDa
• Numéro d’acquisition GenBank: BC040261
• Symbole du gène: CD86
• Identification du gène (NCBI): 942
• Conjugaison: APC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 650 nm / 660 nm
• Forme: Liquide
• Méthode de purification: L'anticorps purifié est conjugué à l'allophycocyanine (APC) dans des conditions optimales. Le conjugué est purifié par chromatographie d'exclusion de taille.
• Tampon de stockage: PBS avec azoture de sodium à 0,1 %.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD86 (also known as B7.2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response.

Publications

Species	Application	Title
human	FC	Cell Death Discov; A positive feedback loop: RAD18-YAP-TGF-β between triple-negative breast cancer and macrophages regulates cancer stemness and progression.; Authors - Xueqi Yan