Anticorps Recombinant de lapin anti-CD86

CD86 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

FC

Conjugaison

Biotin

CloneNo.

GL1

N° de cat : Biotin-FcA65673

Synonymes

B7, B7 2, B7.2, B70, B7-2

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Galerie de données de validation

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  FC experiment of mouse splenocytes using Biotin-FcA65673

  1x10^6 untreated or LPS-treated mouse splenocytes were surface stained with 0.25 ug Biotin Anti-Mouse CD86 (GL1) Rabbit IgG RecAb (Biotin-FcA65673, Clone: GL1) and PE-conjugated streptavidin. Cells were not fixed.

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  FC experiment of mouse splenocytes using Biotin-FcA65673

  1x10^6 LPS-treated mouse splenocytes were surface stained with 0.25 ug Biotin Anti-Mouse CD86 (GL1) Rabbit IgG RecAb (Biotin-FcA65673, Clone: GL1) (red) or 0.25 ug Biotin-conjugated IgG control Rabbit PolyAb (Biotin-30000) (blue), and PE-conjugated streptavidin. 1x10^6 untreated mouse splenocytes were surface stained with 0.25 ug Biotin Anti-Mouse CD86 (GL1) Rabbit IgG RecAb (Biotin-FcA65673, Clone: GL1) (black, dashed), and PE-conjugated streptavidin. Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: LPS-treated mouse splenocytes

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

Biotin-FcA65673 cible CD86 dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: s/o
• Nom complet: CD86 antigen
• Numéro d’acquisition GenBank: BC013807
• Symbole du gène: Cd86
• Identification du gène (NCBI): 12524
• Conjugaison: Biotin
• Excitation/Emission maxima wavelengths: -
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD86 (also known as B7-2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and CTLA-4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with CTLA-4 negatively regulates T-cell activation and diminishes the immune response. (PMID: 7513726; 1847722; 11029388; 27591335)

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