Anticorps Recombinant de lapin anti-CD86

CD86 Recombinant Antibody for IF/ICC, FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

IF/ICC, FC

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230476B8

N° de cat : CL488-98025

Synonymes

230476B8, B7, B7 2, B7.2, B70

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Galerie de données de validation

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  IF Staining of RAW 264.7 using CL488-98025

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using CoraLite® Plus 488 CD86 antibody (CL488-98025, Clone: 230476B8 ) at dilution of 1:200.

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  FC experiment of mouse splenocytes using CL488-98025

  1x10^6 unstimulated (left) or LPS treated (right) mouse splenocytes were surface stained with 0.25ug CoraLite® Plus 488 Anti-Mouse CD86 Rabbit Recombinant Antibody (CL488-98025, Clone:230476B8). Cells were not fixed.

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  FC experiment of mouse splenocytes using CL488-98025

  1x10^6 LPS treated mouse splenocytes were surface stained with 0.25ug CoraLite® Plus 488 Anti-Mouse CD86 Rabbit Recombinant Antibody (CL488-98025, Clone: 230476B8) (red) or Isotype Control (blue). 1x10^6 untreated mouse splenocytes were surface stained with 0.25 ug CoraLite® Plus 488 Anti-Mouse CD86 Rabbit Recombinant Antibody (CL488-98025, Clone: 230476B8) (black, dashed). Cells were not fixed.

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Applications testées

• Résultats positifs en IF/ICC: cellules RAW 264.7,
• Résultats positifs en cytométrie: LPS treated mouse splenocytes,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-98025 cible CD86 dans les applications de IF/ICC, FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD86 Protéine recombinante Eg0802
• Nom complet: CD86 antigen
• Masse moléculaire calculée: 35 kDa
• Numéro d’acquisition GenBank: NM_019388
• Symbole du gène: Cd86
• Identification du gène (NCBI): 12524
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD86 (also known as B7-2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and CTLA-4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with CTLA-4 negatively regulates T-cell activation and diminishes the immune response. (PMID: 7513726; 1847722; 11029388; 27591335)

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