Anticorps Recombinant de lapin anti-CD86

CD86 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

FITC Plus Fluorescent Dye

CloneNo.

BU63

N° de cat : FITC-65514

Synonymes

Activation B7 2 antigen, Activation B7-2 antigen, B7 2, B70, BU63

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Galerie de données de validation

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  FC experiment of human PBMCs using FITC-65514

  1x10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD86 Rabbit Recombinant Antibody (FITC-65514, Clone:BU63) or FITC-conjugated Rabbit IgG control PolyAb (FITC-30000). Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Monocytes were gated.

• 

  FC experiment of human PBMCs using FITC-65514

  1x10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD86 Rabbit Recombinant Antibody (FITC-65514, Clone:BU63) or FITC-conjugated Rabbit IgG control PolyAb (FITC-30000). Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Monocytes were gated.

• 

  FC experiment of human PBMCs using FITC-65514

  1x10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD86 Rabbit Recombinant Antibody (FITC-65514, Clone:BU63) or 5 ul FITC Plus Anti-Human CD86 Mouse Monoclonal Antibody (FITC-65165, Clone:BU63). Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Monocytes were gated.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

FITC-65514 cible CD86 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Lignée cellulaire B-lymphoblastoïde ARH 77
• Nom complet: CD86 molecule
• Masse moléculaire calculée: 329 aa, 38 kDa
• Numéro d’acquisition GenBank: BC040261
• Symbole du gène: CD86
• Identification du gène (NCBI): 942
• Conjugaison: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD86 (also known as B7-2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and CTLA-4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with CTLA-4 negatively regulates T-cell activation and diminishes the immune response. (PMID: 7513726; 1847722; 11029388; 27591335)

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