Anticorps Polyclonal de lapin anti-CD8a

CD8a Polyclonal Antibody for WB, IF-Fro, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris et plus (1)

Applications

WB, IF-Fro, ELISA

Conjugaison

Non conjugué

Publications(23)

N° de cat : 29896-1-AP

Synonymes

CD8, CD8 antigen, alpha chain, CD8α, Ly 2, Ly 35

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Galerie de données de validation

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  WB analysis using 29896-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29896-1-AP (CD8a antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse spleen using 29896-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse spleen tissue using 29896-1-AP (CD8a antibody) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu splénique de souris, tissu de thymus de souris
• Résultats positifs en IF-Fro: tissu splénique de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunofluorescence (IF)-FRO: IF-FRO : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 6 publications below

Informations sur le produit

29896-1-AP cible CD8a dans les applications de WB, IF-Fro, ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD8a Protéine recombinante Ag31942
• Nom complet: CD8 antigen, alpha chain
• Masse moléculaire calculée: 27KD
• Poids moléculaire observé: 32-35 kDa
• Numéro d’acquisition GenBank: NM_001081110
• Symbole du gène: Cd8a
• Identification du gène (NCBI): 12525
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD8 is a transmembrane glycoprotein that is predominantly expressed on the surface of cytotoxic T cells, and can also be found on natural killer cells, cortical thymocytes, and dendritic cells. CD8 is composed of two disulfide-linked chains and can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).

Publications

Species	Application	Title
mouse		J Nanobiotechnology; Oxygen-delivery nanoparticles enhanced immunotherapy efficacy monitored by granzyme B PET imaging in malignant tumors; Authors - Xingyi Wang
human		Front Immunol; Machine learning-based identification of tumor-infiltrating immune cell-associated model with appealing implications in improving prognosis and immunotherapy response in bladder cancer patients; Authors - Hualin Chen
mouse	IF	Int Immunopharmacol; Triggering receptor expressed on myeloid cells-1 aggravates obliterative bronchiolitis via enhancing the proinflammatory phenotype of macrophages; Authors - Junhao Wan
mouse		Bioorg Chem; Synthesis and immunotherapy efficacy of a PD-L1 small-molecule inhibitor combined with its 131I-iodide labelled isostructural compound; Authors - Gaochao Lv
mouse		Int J Pharm X; Topical transdermal administration of lenalidomide nanosuspensions-based hydrogels against melanoma: In vitro and in vivo studies; Authors - Mengdi Zhang
mouse	IF	J Transl Med; Escherichia coli combination with PD-1 blockade synergistically enhances immunotherapy in glioblastoma multiforme by regulating the immune cells; Authors - Guochen Li

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