Anticorps Monoclonal anti-CD8a

CD8a Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

127

CloneNo.

1G2B10

N° de cat : 66868-1-Ig

Synonymes

CD8, CD8A, CD8a molecule, Leu2, MAL, p32

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Galerie de données de validation

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  WB analysis of MOLT-4 using 66868-1-Ig

  MOLT-4 cells were subjected to SDS PAGE followed by western blot with 66868-1-Ig (CD8a antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human peripheral blood leukocyte using 66868-1-Ig

  Human peripheral blood leukocyte cells were subjected to SDS PAGE followed by western blot with 66868-1-Ig (CD8a antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 66868-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66868-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66868-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66868-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 66868-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000 (under 10x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

• 

  IHC staining of human pancreas cancer using 66868-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000 (under 40x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

• 

  IHC staining of human pancreas cancer using 66868-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 66868-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66868-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66868-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66868-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66868-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66868-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66868-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66868-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66868-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66868-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66868-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 66868-1-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:4000 (under 10x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

• 

  IHC staining of human appendicitis using 66868-1-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:4000 (under 40x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

• 

  IF Staining of human tonsillitis using 66868-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD8a antibody (66868-1-Ig, Clone: 1G2B10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human tonsillitis using 66868-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD8a antibody (66868-1-Ig, Clone: 1G2B10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules MOLT-4, leucocyte de sang périphérique humain
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu d'appendicite humain, tissu de cancer du côlon humain, tissu de cancer du pancréas humain, tissu de cancer du poumon humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu d'amygdalite humain,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:10000-1:40000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below
• IHC: See 77 publications below
• IF: See 43 publications below
• FC: See 4 publications below

Informations sur le produit

66868-1-Ig cible CD8a dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CD8a Protéine recombinante Ag11111
• Nom complet: CD8a molecule
• Masse moléculaire calculée: 235 aa, 26 kDa
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: BC025715
• Symbole du gène: CD8A
• Identification du gène (NCBI): 925
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD8 is a transmembrane glycoprotein that is predominantly expressed on the surface of cytotoxic T cells, and can also be found on natural killer cells, cortical thymocytes, and dendritic cells. CD8 serves as a co-receptor for the T cell receptor (TCR). Both CD8 and TCR recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. CD8 plays a role in T cell development and activation of mature T cells. This antibody is raised against 23-183 aa of human CD8a, the alpha chain of CD8 molecule.

Publications

Species	Application	Title
	IF	ACS Nano; Tailoring Chemoimmunostimulant Bioscaffolds for Inhibiting Tumor Growth and Metastasis after Incomplete Microwave Ablation.; Authors - Yuting Shen
human	IF	J Med Virol; Combination of novel oncolytic herpesvirus with paclitaxel as an efficient strategy for breast cancer therapy; Authors - Xinyue Deng
human	IF	Cell Death Differ; METTL3 inhibition induced by M2 macrophage-derived extracellular vesicles drives anti-PD-1 therapy resistance via M6A-CD70-mediated immune suppression in thyroid cancer; Authors - Junya Ning
human	IHC	Nat Commun; Single-cell transcriptomics links malignant T cells to the tumor immune landscape in cutaneous T cell lymphoma.; Authors - Xiangjun Liu
human	IF	J Exp Clin Cancer Res; FAM171B stabilizes vimentin and enhances CCL2-mediated TAM infiltration to promote bladder cancer progression; Authors - Wei-Min Hu
human	IF,FC	J Immunother Cancer; Interleukin-10 induces expression of CD39 on CD8+T cells to potentiate anti-PD1 efficacy in EGFR-mutated non-small cell lung cancer; Authors - Meng Qiao