Anticorps Monoclonal anti-CD8a

CD8a Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, FC

Conjugaison

Non conjugué

Publications

4

CloneNo.

RPA-T8

N° de cat : 65144-1-Ig

Synonymes

CD8, CD8A, CD8a molecule, Leu2, MAL, p32

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Galerie de données de validation

• 

  FC experiment of human peripheral blood lymphocytes using 65144-1-Ig

  1X10^6 human peripheral blood lymphocytes were surface stained with 0.5 ug Anti-Human CD8a (65144-1-Ig, Clone:RPA-T8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), or stained with 0.5 ug isotype control antibody and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (black). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: lymphocytes de sang périphérique humain,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 3 publications below

Informations sur le produit

65144-1-Ig cible CD8a dans les applications de WB, IHC, FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, porc
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: s/o
• Nom complet: CD8a molecule
• Masse moléculaire calculée: 235 aa, 26 kDa
• Numéro d’acquisition GenBank: BC025715
• Symbole du gène: CD8A
• Identification du gène (NCBI): 925
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: Tampon à base de phosphate avec azoture de sodium à 0,09 %, pH 7,2.
• Conditions de stockage: Stocker à une température comprise entre 2 °C et 8 °C.

Informations générales

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).

Publications

Species	Application	Title
human	WB	Cancers (Basel); Inhibition of DCLK1 with DCLK1-IN-1 Suppresses Renal Cell Carcinoma Invasion and Stemness and Promotes Cytotoxic T-Cell-Mediated Anti-Tumor Immunity.; Authors - Ling Ding
human	IHC	Front Oncol; Comprehensive Characterization of Immune Landscape Based on Epithelial-Mesenchymal Transition Signature in OSCC: Implication for Prognosis and Immunotherapy.; Authors - Si-Yuan Zhang
human	IHC	Front Oncol; Typing and modeling of hepatocellular carcinoma based on disulfidptosis-related amino acid metabolism genes for predicting prognosis and guiding individualized treatment; Authors - Xuenuo Chen
pig	IHC	J Virol; Combinational Deletions of MGF360-9L and MGF505-7R Attenuated Highly Virulent African Swine Fever Virus and Conferred Protection against Homologous Challenge.; Authors - Mingyang Ding