Anticorps Recombinant de lapin anti-CD8a

CD8a Recombinant Antibody for FC

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

CloneNo.

OKT8

N° de cat : 65604-1-PBS

Synonymes

CD8, p32, OKT8, MAL, Leu2

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Galerie de données de validation

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  FC experiment of human PBMCs using 65604-1-MR (same clone as 65604-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD8a (OKT8) Mouse IgG2a Recombinant Antibody (65604-1-MR, Clone:OKT8) or 0.25 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4), and PE-conjugated Goat Anti-Mouse IgG. Cells were then stained with APC Anti-Human CD3. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65604-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65604-1-MR (same clone as 65604-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD8a (OKT8) Mouse IgG2a Recombinant Antibody (65604-1-MR, Clone: OKT8) (red) or 0.25 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue), and PE-conjugated Goat Anti-Mouse IgG. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65604-1-PBS in a different storage buffer formulation.

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Informations sur le produit

65604-1-PBS cible CD8a dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Thymocytes humains
• Nom complet: CD8a molecule
• Masse moléculaire calculée: 235 aa, 26 kDa
• Numéro d’acquisition GenBank: BC025715
• Symbole du gène: CD8A
• Identification du gène (NCBI): 925
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C.

Informations générales

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells, on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T-cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).

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