Anticorps Recombinant de lapin anti-CD8a

CD8a Recombinant Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242879G4

N° de cat : 85977-3-PBS

Synonymes

CD8, CD8 antigen, alpha chain, CD8α, Ly 2, Ly 35

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Galerie de données de validation

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  WB analysis of mouse thymus using 85977-3-RR (same clone as 85977-3-PBS)

  Mouse thymus tissue was subjected to SDS PAGE followed by western blot with 85977-3-RR (CD8A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85977-3-PBS in a different storage buffer formulation.

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Informations sur le produit

85977-3-PBS cible CD8a dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD8a Protéine recombinante Eg3265
• Nom complet: CD8 antigen, alpha chain
• Masse moléculaire calculée: 27 kDa
• Poids moléculaire observé: 32-35 kDa
• Numéro d’acquisition GenBank: NM_001081110.2
• Symbole du gène: Cd8a
• Identification du gène (NCBI): 12525
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD8 is a transmembrane glycoprotein that is predominantly expressed on the surface of cytotoxic T cells, and can also be found on natural killer cells, cortical thymocytes, and dendritic cells. CD8 is composed of two disulfide-linked chains and can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells, on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).

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