Anticorps Recombinant de lapin anti-CD8a

CD8a Recombinant Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

CloneNo.

242879E1

N° de cat : 85977-4-RR

Synonymes

CD8, CD8 antigen, alpha chain, CD8α, Ly 2, Ly 35

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Galerie de données de validation

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  WB analysis using 85977-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85977-4-RR (CD8A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse thymus using 85977-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 85977-4-RR (CD8a antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse thymus using 85977-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 85977-4-RR (CD8a antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 85977-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 85977-4-RR (CD8a antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 85977-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 85977-4-RR (CD8a antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse spleen using 85977-4-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD8a antibody (85977-4-RR, Clone: 242879E1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse spleen using 85977-4-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD8a antibody (85977-4-RR, Clone: 242879E1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse thymus using 85977-4-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse thymus tissue using CD8a antibody (85977-4-RR, Clone: 242879E1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse thymus using 85977-4-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse thymus tissue using CD8a antibody (85977-4-RR, Clone: 242879E1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu splénique de souris, tissu de thymus de souris
• Résultats positifs en IHC: tissu de thymus de souris, tissu splénique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu splénique de souris, tissu de thymus de souris

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85977-4-RR cible CD8a dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD8a Protéine recombinante Eg3265
• Nom complet: CD8 antigen, alpha chain
• Masse moléculaire calculée: 27 kDa
• Poids moléculaire observé: 30-35 kDa
• Numéro d’acquisition GenBank: NM_001081110.2
• Symbole du gène: Cd8a
• Identification du gène (NCBI): 12525
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD8 is a transmembrane glycoprotein that is predominantly expressed on the surface of cytotoxic T cells, and can also be found on natural killer cells, cortical thymocytes, and dendritic cells. CD8 is composed of two disulfide-linked chains and can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).

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