Anticorps Recombinant de lapin anti-CD8a

CD8a Recombinant Antibody for IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

243018F10

N° de cat : 86063-2-RR

Synonymes

CD8, CD8a molecule, Leu2, MAL, p32

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Galerie de données de validation

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  IHC staining of human tonsillitis using 86063-2-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 86063-2-RR (CD8a antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 86063-2-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 86063-2-RR (CD8a antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary cancer using 86063-2-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 86063-2-RR (CD8a antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 86063-2-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 86063-2-RR (CD8a antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 86063-2-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 86063-2-RR (CD8a antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human rectal cancer using 86063-2-RR

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 86063-2-RR (CD8a antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human rectal cancer using 86063-2-RR

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 86063-2-RR (CD8a antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocellular cancer using 86063-2-RR

  Immunohistochemical analysis of paraffin-embedded human hepatocellular cancer slide using 86063-2-RR (CD8a antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human hepatocellular cancer using 86063-2-RR

  Immunohistochemical analysis of paraffin-embedded human hepatocellular cancer slide using 86063-2-RR (CD8a antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 86063-2-RR

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 86063-2-RR (CD8a antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 86063-2-RR

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 86063-2-RR (CD8a antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 86063-2-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 86063-2-RR (CD8a antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary cancer using 86063-2-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 86063-2-RR (CD8a antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human tonsillitis using 86063-2-RR

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD8a antibody (86063-2-RR, Clone: 243018F10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of human tonsillitis using 86063-2-RR

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD8a antibody (86063-2-RR, Clone: 243018F10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  Affinity and Kinetic Characterization of 86063-2-RR

  Biolayer interferometry (BLl) kinetic assays of 86063-2-RR against Human CD8a were performed. The affinity constant is 13.2 nM.

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Applications testées

• Résultats positifs en IHC: tissu d'amygdalite humain, tissu de cancer de l'estomac humain, tissu de cancer du sein humain, tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: tissu d'amygdalite humain,

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86063-2-RR cible CD8a dans les applications de IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD8a Protéine recombinante Eg0819
• Nom complet: CD8a molecule
• Masse moléculaire calculée: 26 kDa
• Numéro d’acquisition GenBank: NM_001145873.1
• Symbole du gène: CD8A
• Identification du gène (NCBI): 925
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD8 is a transmembrane glycoprotein that is predominantly expressed on the surface of cytotoxic T cells, and can also be found on natural killer cells, cortical thymocytes, and dendritic cells. CD8 serves as a co-receptor for the T cell receptor (TCR). Both CD8 and TCR recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. CD8 plays a role in T cell development and activation of mature T cells. This antibody is raised against 22-182 aa of human CD8a, the alpha chain of CD8 molecule.

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