Anticorps Recombinant de lapin anti-CD8a

CD8a Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

FC

Conjugaison

Non conjugué

CloneNo.

242879C10

N° de cat : 98472-2-PBS

Synonymes

CD8, CD8 antigen, alpha chain, CD8α, Ly 2, Ly 35

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Galerie de données de validation

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  FC experiment of mouse splenocytes using 98472-2-RR (same clone as 98472-2-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD8a Rabbit RecAb (98472-2-RR, Clone: 242879C10) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Mouse CD3. Cells were not fixed. This data was developed using the same antibody clone with 98472-2-PBS in a different storage buffer formulation.

• 

  FC experiment of mouse splenocytes using 98472-2-RR (same clone as 98472-2-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD8a Rabbit RecAb (98472-2-RR, Clone: 242879C10) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98472-2-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 98472-2-RR

  Biolayer interferometry (BLl) kinetic assays of 98472-2-RR against Mouse CD8a were performed. The affinity constant is below 1 pM.

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Informations sur le produit

98472-2-PBS cible CD8a dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD8a Protéine recombinante Eg3265
• Nom complet: CD8 antigen, alpha chain
• Masse moléculaire calculée: 27 kDa
• Numéro d’acquisition GenBank: NM_001081110.2
• Symbole du gène: Cd8a
• Identification du gène (NCBI): 12525
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C.

Informations générales

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells, on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).

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