Anticorps Recombinant de lapin anti-CD8a

CD8a Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

APC Fluorescent Dye

CloneNo.

OKT8

Avis (1)

N° de cat : APC-FcA65506

Synonymes

CD8, p32, OKT8, MAL, Leu2

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Galerie de données de validation

• 

  FC experiment of human PBMCs using APC-FcA65506

  1x10^6 human PBMCs were surface stained with APC Anti-Human CD4 and 5 ul PE Anti-Human CD8a (OKT8) Rabbit IgG Recombinant Antibody (PE-FcA65506, Clone: OKT8) or PE Rabbit IgG Isotype Control Recombinant Antibody (PE-FcA98136, Clone: 240953C9). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines,

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

APC-FcA65506 cible CD8a dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Thymocytes humains
• Nom complet: CD8a molecule
• Masse moléculaire calculée: 235 aa, 26 kDa
• Numéro d’acquisition GenBank: BC025715
• Symbole du gène: CD8A
• Identification du gène (NCBI): 925
• Conjugaison: APC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 650 nm / 660 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells, on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T-cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Eduardo (Verified Customer) (07-02-2025)

The titration results for the anti-CD8 antibody conjugated to APC show excellent performance across a range of concentrations. Notably, the signal intensity increases proportionally with antibody concentration, with clear separation between positive and negative populations beginning as early as the 0.25:150 dilution. Optimal staining appears to be achieved at the 2:150 and 4:150 dilutions, with 4:150 offering improved resolution between CD8^dim and CD8^bright populations due to enhanced signal separation.

• Applications: Flow Cytometry
• Primary Antibody Dilution: 4:150 , 2:150 , 1:150, 0.5:150, 0.25:150
• Cell Tissue Type: Human PBMCs

APC CD8a Antibody Flow Cytometry validation (4:150 , 2:150 , 1:150, 0.5:150, 0.25:150 dilution) in Human PBMCs (Cat no:APC-FcA65506)

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