Anticorps Recombinant de lapin anti-CD8a

CD8a Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

Biotin

CloneNo.

OKT8

N° de cat : Biotin-FcA65506

Synonymes

CD8, CD8a molecule, Leu2, MAL, OKT8

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Galerie de données de validation

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  FC experiment of human PBMCs using Biotin-FcA65506

  1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD8a (OKT8) Rabbit IgG RecAb (Biotin-FcA65506, Clone: OKT8) or Biotin Rabbit IgG Isotype Control RecAb (Biotin-FcA98136, Clone: 240953C9), and PE-conjugated streptavidin. Cells were then stained with FcZero-rAb™ APC Anti-Human CD4 Rabbit Recombinant Antibody (APC-FcA98042, Clone: 240427E12). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

Biotin-FcA65506 cible CD8a dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Thymocytes humains
• Nom complet: CD8a molecule
• Masse moléculaire calculée: 235 aa, 26 kDa
• Numéro d’acquisition GenBank: BC025715
• Symbole du gène: CD8A
• Identification du gène (NCBI): 925
• Conjugaison: Biotin
• Excitation/Emission maxima wavelengths: -
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells, on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T-cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).

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