Anticorps Monoclonal anti-CD8a

CD8a Monoclonal Antibody for IF-P

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

IHC, IF-P

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

Publications(3)

CloneNo.

1G2B10

N° de cat : CL488-66868

Synonymes

CD8, 1G2B10, p32, MAL, Leu2

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Galerie de données de validation

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  IF Staining of human tonsillitis using CL488-66868

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 488 CD8a antibody (CL488-66868, Clone: 1G2B10 ) at dilution of 1:200, CoraLite®594 CD11c/Integrin Alpha X antibody (CL594-60258, Clone: 2F1C10, red).

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  IF Staining of human tonsillitis using CL488-66868

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL488-66868 (CD8a antibody) at dilution of 1:100.

• 

  IF Staining of human tonsillitis using CL488-66868

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 488 CD8a antibody (CL488-66868, Clone: 1G2B10 ) at dilution of 1:200.

• 

  IF Staining of human tonsillitis using CL488-66868

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 488 CD8a antibody (CL488-66868, Clone: 1G2B10 ) at dilution of 1:200.

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Applications testées

• Résultats positifs en IF-P: tissu d'amygdalite humain,

Dilution recommandée

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

CL488-66868 cible CD8a dans les applications de IHC, IF-P et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CD8a Protéine recombinante Ag11111
• Nom complet: CD8a molecule
• Masse moléculaire calculée: 235 aa, 26 kDa
• Numéro d’acquisition GenBank: BC025715
• Symbole du gène: CD8A
• Identification du gène (NCBI): 925
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD8 is a transmembrane glycoprotein that is predominantly expressed on the surface of cytotoxic T cells, and can also be found on natural killer cells, cortical thymocytes, and dendritic cells. CD8 serves as a co-receptor for the T cell receptor (TCR). Both CD8 and TCR recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. CD8 plays a role in T cell development and activation of mature T cells. This antibody is raised against 23-183 aa of human CD8a, the alpha chain of CD8 molecule, and is conjugated with CL488, Ex/Em 488 nm/515 nm.

Publications

Species	Application	Title
	IHC	FASEB J; Tertiary lymphoid structures: Associated multiple immune cells and analysis their formation in hepatocellular carcinoma; Authors - Ye Nie
mouse	IF	J Cardiovasc Pharmacol; Treg/Th17 Ratio Regulation May Play an Important Role in Epigallocatechin-3-Gallate-Mediated Attenuation of Increased Afterload-Induced Cardiac Hypertrophy; Authors - Min Luo
		J Ethnopharmacol; Huangqin Tang Alleviates Colitis-associated Colorectal Cancer via Amino Acids Homeostasisand PI3K/AKT/mTOR Pathway Modulation; Authors - Dunfang Wang

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