Anticorps Recombinant de lapin anti-CD8b

CD8b Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

FC

Conjugaison

Non conjugué

CloneNo.

250480F2

N° de cat : 98461-3-RR

Synonymes

Cd8b1, Ly-3, Lymphocyte antigen 3, Lyt3, Lyt-3

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Galerie de données de validation

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  FC experiment of mouse splenocytes using 98461-3-RR

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD8b Rabbit RecAb (98461-3-RR, Clone: 250480F2) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Mouse CD3 (17A2) (APC-65077, Clone: 17A2). Cells were not fixed.

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  FC experiment of mouse splenocytes using 98461-3-RR

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD8b Rabbit RecAb (98461-3-RR, Clone: 250480F2) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: splénocytes de souris,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98461-3-RR cible CD8b dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD8b Protéine recombinante Eg4275
• Nom complet: CD8 antigen, beta chain 1
• Masse moléculaire calculée: 24 kDa
• Numéro d’acquisition GenBank: NM_009858.2
• Symbole du gène: Cd8b1
• Identification du gène (NCBI): 12526
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

CD8b (T-cell surface glycoprotein CD8 beta chain) is an integral membrane glycoprotein that forms disulfide-linked heterodimers with CD8a (CD8 alpha chain). The CD8 alpha/beta heterodimer is the predominant CD8 complex expressed on the cell surface (PMID: 1534146; 2111591). CD8 is a transmembrane glycoprotein predominantly expressed on the surface of cytotoxic T cells and can also be found on natural killer cells, cortical thymocytes, and dendritic cells. CD8 serves as a co-receptor for the T cell receptor (TCR). Both CD8 and TCR recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. CD8 plays a role in T cell development and activation of mature T cells.

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