Anticorps Polyclonal de lapin anti-CD9

• Phare
• Validé par KD/KO

CD9 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (9)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(341)

N° de cat : 20597-1-AP

Synonymes

5H9, 5H9 antigen, BA2, BTCC 1, CD9 antigen

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Galerie de données de validation

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  WB analysis using 20597-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20597-1-AP (CD9 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 20597-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20597-1-AP (CD9 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 20597-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20597-1-AP (CD9 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary cancer using 20597-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 20597-1-AP (CD9 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary cancer using 20597-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 20597-1-AP (CD9 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 20597-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20597-1-AP (CD9 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 20597-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20597-1-AP (CD9 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human endometrial cancer using 20597-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 20597-1-AP (CD9 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human endometrial cancer using 20597-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 20597-1-AP (CD9 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 20597-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CD9 antibody (20597-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HCT 116, cellules HeLa, cellules L02
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de cancer de l'endomètre humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 325 publications below
• IHC: See 7 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• FC: See 1 publications below

Informations sur le produit

20597-1-AP cible CD9 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Chèvre, Humain, Lapin, poisson-zèbre, porc, poulet, singe, souris, Chauve-souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD9 Protéine recombinante Ag14546
• Nom complet: CD9 molecule
• Masse moléculaire calculée: 228 aa, 25 kDa
• Poids moléculaire observé: 23-30 kDa
• Numéro d’acquisition GenBank: BC011988
• Symbole du gène: CD9
• Identification du gène (NCBI): 928
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The cell-surface molecule CD9, a member of the transmembrane-4 superfamily, interacts with the integrin family and other membrane proteins, and is postulated to participate in cell migration and adhesion. Expression of CD9 enhances membrane fusion between muscle cells and promotes viral infection in some cells (PMID:10459022). It is often used as a mesenchymal stem cell marker (PMID:18005405). The CD9 antigen appears to be a 227-amino acid molecule with four hydrophobic domains and one N-glycosylation site (PMID: 1840589). This antibody detects bands of 23-30 kDa, it may be due to the difference of glycosylations (PMID: 8701996).

Publications

Species	Application	Title
mouse	WB	Signal Transduct Target Ther; Circulating tumor cells shielded with extracellular vesicle-derived CD45 evade T cell attack to enable metastasis; Authors - Chuan Yang
human	WB	Gastroenterology; PTEN deficiency facilitates exosome secretion and metastasis in cholangiocarcinoma by impairing TFEB-mediated lysosome biogenesis; Authors - Tian-Yi Jiang
human	WB	Cell Res; In vivo self-assembled small RNAs as a new generation of RNAi therapeutics.; Authors - Zheng Fu
	WB	Bioact Mater; Profibrogenic macrophage-targeted delivery of mitochondrial protector via exosome formula for alleviating pulmonary fibrosis; Authors - Wei Zhang
rat	WB,IF	Nat Commun; Injectable ECM-mimetic dynamic hydrogels abolish ferroptosis-induced post-discectomy herniation through delivering nucleus pulposus progenitor cell-derived exosomes; Authors - Wenkai Wang
human	WB	J Extracell Vesicles; Quantification of urinary podocyte-derived migrasomes for the diagnosis of kidney disease; Authors - Rong Yang

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