Anticorps Monoclonal anti-CD9

• Phare
• Validé par KD/KO

CD9 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, PLA, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

76

CloneNo.

4H7B9

Avis (1)

N° de cat : 60232-1-Ig

Synonymes

5H9, 5H9 antigen, BA2, BTCC 1, CD9, CD9 antigen, CD9 molecule, Cell and organelle markers, DRAP 27, Exosome marker, GIG2, Leukocyte antigen MIC3, MIC3, Motility related protein, MRP 1, P24, Tetraspanin 29, Tspan 29, TSPAN29

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Galerie de données de validation

• 

  WB analysis of HeLa using 60232-1-Ig

  WB result of CD9 antibody (60232-1-Ig, 1:2000) with si-Control and si-CD9 transfected HeLa cells.

• 

  WB analysis using 60232-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60232-1-Ig (CD9 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  IHC staining of human ovary tumor using 60232-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60232-1-Ig (CD9 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 60232-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60232-1-Ig (CD9 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 60232-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60232-1-Ig (CD9 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 60232-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60232-1-Ig (CD9 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 60232-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60232-1-Ig (CD9 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 60232-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60232-1-Ig (CD9 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 60232-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60232-1-Ig (CD9 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 60232-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60232-1-Ig (CD9 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human ovary tumor using 60232-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using CD9 antibody (60232-1-Ig, Clone: 4H7B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human ovary tumor using 60232-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using CD9 antibody (60232-1-Ig, Clone: 4H7B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human breast cancer using 60232-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CD9 antibody (60232-1-Ig, Clone: 4H7B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human breast cancer using 60232-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CD9 antibody (60232-1-Ig, Clone: 4H7B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human lung cancer using 60232-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CD9 antibody (60232-1-Ig, Clone: 4H7B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human lung cancer using 60232-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CD9 antibody (60232-1-Ig, Clone: 4H7B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of HeLa using 60232-1-Ig

  1X10^6 HeLa cells were stained with 0.2 ug Anti-Human CD9 (60232-1-Ig, Clone:4H7B9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), or 0.2 ug isotype control (black). Cells were fixed with 4% PFA.

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HeLa
• Résultats positifs en IHC: tissu de tumeur ovarienne humain, tissu d'amygdalite humain, tissu de cancer du côlon humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu de cancer du sein humain, tissu de cancer du poumon humain, tissu de tumeur ovarienne humain
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 69 publications below
• IHC: See 1 publications below
• IF: See 6 publications below
• FC: See 1 publications below

Informations sur le produit

60232-1-Ig cible CD9 dans les applications de WB, PLA, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CD9 Protéine recombinante Ag14529
• Nom complet: CD9 molecule
• Masse moléculaire calculée: 228 aa, 25 kDa
• Poids moléculaire observé: 23-27 kDa
• Numéro d’acquisition GenBank: BC011988
• Symbole du gène: CD9
• Identification du gène (NCBI): 928
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The cell-surface molecule CD9, a member of the transmembrane-4 superfamily, interacts with the integrin family and other membrane proteins, and is postulated to participate in cell migration and adhesion. Expression of CD9 enhances membrane fusion between muscle cells and promotes viral infection in some cells (PMID:10459022). It is often used as a mesenchymal stem cell marker (PMID:18005405). CD9 is also known as the p24 antigen besides MIC3, TSPAN29 because it is a protein of molecular weight 24 kD. The CD9 antigen appears to be a 227-amino acid molecule with 4 hydrophobic domains and 1 N-glycosylation site.

Publications

Species	Application	Title
human	WB	Brain; Extracellular vesicle biomarkers for complement dysfunction in schizophrenia; Authors - Ting Xue
human	WB	Mol Ther; Single-cell dissection of cellular and molecular features underlying mesenchymal stem cell therapy in ischemic acute kidney injury; Authors - Wenjuan Wang
human	WB	Small Methods; Highly Sensitive Exosome Detection for Early Diagnosis of Pancreatic Cancer Using Immunoassay Based on Hierarchical Surface-Enhanced Raman Scattering Substrate.; Authors - Juan Li
human	WB	Biomaterials; Urinary exosomes-based Engineered Nanovectors for Homologously Targeted Chemo-Chemodynamic Prostate Cancer Therapy via abrogating IGFR/AKT/NF-kB/IkB signaling.; Authors - Shaojun Pan
human	WB	Pharmacol Res; Plasma extracellular vesicle long RNAs predict response to neoadjuvant immunotherapy and survival in patients with non-small cell lung cancer; Authors - Wei Guo
	WB	Cell Death Dis; Ficolin B secreted by alveolar macrophage exosomes exacerbates bleomycin-induced lung injury via ferroptosis through the cGAS-STING signaling pathway; Authors - Xu Wu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Guorong (Verified Customer) (03-22-2022)

Very weak bands with strong background were detected at 25 kDa

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: ARPE-19

CD9 Antibody Western Blot validation (1:5000 dilution) in ARPE-19 (Cat no:60232-1-Ig)

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