Anticorps Recombinant de lapin anti-CD9

CD9 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

240830A8

N° de cat : 84142-1-PBS

Synonymes

240830A8, 5H9, 5H9 antigen, BA2, BTCC 1

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Galerie de données de validation

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  WB analysis using 84142-1-RR (same clone as 84142-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84142-1-RR (CD9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84142-1-PBS in a different storage buffer formulation.

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  WB analysis using 84142-1-RR (same clone as 84142-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84142-1-RR (CD9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84142-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 84142-1-RR (same clone as 84142-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84142-1-RR (CD9 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84142-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 84142-1-RR (same clone as 84142-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84142-1-RR (CD9 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84142-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 84142-1-RR (same clone as 84142-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CD9 antibody (84142-1-RR, Clone: 240830A8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84142-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84142-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84142-1-RR against Human CD9 were performed. The affinity constant is 6.21 nM.

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Informations sur le produit

84142-1-PBS cible CD9 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD9 Protéine recombinante Eg1037
• Nom complet: CD9 molecule
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 23 kDa
• Numéro d’acquisition GenBank: NM_001769.4
• Symbole du gène: CD9
• Identification du gène (NCBI): 928
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The cell-surface molecule CD9, a member of the transmembrane-4 superfamily, interacts with the integrin family and other membrane proteins, and is postulated to participate in cell migration and adhesion. Expression of CD9 enhances membrane fusion between muscle cells and promotes viral infection in some cells (PMID:10459022). It is often used as a mesenchymal stem cell marker (PMID:18005405). The CD9 antigen appears to be a 227-amino acid molecule with four hydrophobic domains and one N-glycosylation site (PMID: 1840589). This antibody detects bands of 23-30 kDa, which may be due to the difference in glycosylations (PMID: 8701996).

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