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Anticorps Recombinant de lapin anti-CD9

CD9 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

240830A6

N° de cat : 84142-2-PBS

Synonymes

CD9 antigen, BTCC 1, BA2, 5H9 antigen, 5H9

Galerie de données de validation

Filter:
  • Western Blot (WB) analysis of various lysates using CD9 Recombinant antibody (84142-2-RR)

    WB analysis using 84142-2-RR (same clone as 84142-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84142-2-RR (CD9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84142-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CD9 Recombinant antibody (84142-2-RR)

    IF Staining of MCF-7 using 84142-2-RR (same clone as 84142-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CD9 antibody (84142-2-RR, Clone: 240830A6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84142-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84142-2-RR

    Affinity and Kinetic Characterization of 84142-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84142-2-RR against Human CD9 were performed. The affinity constant is 1.57 nM.

Informations sur le produit

84142-2-PBS cible CD9 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Lapin / IgG
Clonalité Recombinant
Type Anticorps
Immunogène Protéine recombinante
Nom complet CD9 molecule
Masse moléculaire calculée25 kDa
Poids moléculaire observé23 kDa
Numéro d’acquisition GenBankNM_001769.4
Symbole du gène CD9
Identification du gène (NCBI) 928
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Protein A purfication
Tampon de stockage PBS only
Conditions de stockageStore at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The cell-surface molecule CD9, a member of the transmembrane-4 superfamily, interacts with the integrin family and other membrane proteins and is postulated to participate in cell migration and adhesion. Expression of CD9 enhances membrane fusion between muscle cells and promotes viral infection in some cells (PMID:10459022). It is often used as a mesenchymal stem cell marker (PMID:18005405). The CD9 antigen appears to be a 227-amino acid molecule with four hydrophobic domains and one N-glycosylation site (PMID: 1840589). This antibody detects bands of 23-30 kDa, which may be due to the difference in glycosylations (PMID: 8701996).

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