Anticorps Recombinant de lapin anti-CD9

CD9 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

240830A6

N° de cat : 84142-2-RR

Synonymes

CD9 antigen, BTCC 1, BA2, 5H9 antigen, 5H9

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Galerie de données de validation

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  WB analysis using 84142-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84142-2-RR (CD9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 84142-2-RR

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CD9 antibody (84142-2-RR, Clone: 240830A6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84142-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84142-2-RR against Human CD9 were performed. The affinity constant is 1.57 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules L02, tissu placentaire humain
• Résultats positifs en IF/ICC: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84142-2-RR cible CD9 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: CD9 molecule
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 23 kDa
• Numéro d’acquisition GenBank: NM_001769.4
• Symbole du gène: CD9
• Identification du gène (NCBI): 928
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The cell-surface molecule CD9, a member of the transmembrane-4 superfamily, interacts with the integrin family and other membrane proteins and is postulated to participate in cell migration and adhesion. Expression of CD9 enhances membrane fusion between muscle cells and promotes viral infection in some cells (PMID:10459022). It is often used as a mesenchymal stem cell marker (PMID:18005405). The CD9 antigen appears to be a 227-amino acid molecule with four hydrophobic domains and one N-glycosylation site (PMID: 1840589). This antibody detects bands of 23-30 kDa, which may be due to the difference in glycosylations (PMID: 8701996).

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