Anticorps Recombinant de lapin anti-CD9

CD9 Recombinant Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

CloneNo.

242432B5

N° de cat : 84801-13-RR

Synonymes

CD9 antigen, MIC3, P24, Tspan 29, TSPAN29

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Galerie de données de validation

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  WB analysis using 84801-13-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84801-13-RR (CD9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse spleen using 84801-13-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84801-13-RR (CD9 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 84801-13-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84801-13-RR (CD9 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 84801-13-RR

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84801-13-RR (CD9 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 84801-13-RR

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84801-13-RR (CD9 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat spleen using 84801-13-RR

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 84801-13-RR (CD9 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat spleen using 84801-13-RR

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 84801-13-RR (CD9 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 84801-13-RR

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 84801-13-RR (CD9 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 84801-13-RR

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 84801-13-RR (CD9 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse spleen using 84801-13-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD9 antibody (84801-13-RR, Clone: 242432B5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: J774A.1 cells, cellules RAW 264.7, tissu splénique de souris
• Résultats positifs en IHC: tissu splénique de souris, tissu de cancer de la prostate humain, tissu rénal de souris, tissu splénique de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu splénique de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84801-13-RR cible CD9 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD9 Protéine recombinante Eg1397
• Nom complet: CD9 antigen
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 23 kDa
• Numéro d’acquisition GenBank: NM_007657.3
• Symbole du gène: Cd9
• Identification du gène (NCBI): 12527
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD9, also known as Tspan-29, p24 or MIC3, is a member of the tetraspanin superfamily (PMID: 1879540). It is expressed on a large variety of hematopoietic and non-hematopoietic cells, such as stromal cells, megakaryocytes, platelets, B and T lymphocytes, dendritic cells, endothelial cells, mast cells, eosinophils, and basophils (PMID: 30356731). CD9 interacts with the integrin family and other membrane proteins, and is involved in cell adhesion, cell motility and tumor metastasis (PMID: 8478605; 21428940; 25805926). Expression of CD9 enhances membrane fusion between muscle cells and supports myotube maintenance (PMID:10459022). On oocytes, CD9 is hypothesized to play a role in the fertilization of mammals (PMID: 25536312).

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