Anticorps Recombinant de lapin anti-CD9

CD9 Recombinant Antibody for IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242432C2

N° de cat : 84801-4-PBS

Synonymes

242432C2, CD9 antigen, MIC3, P24, Tspan 29

---

Galerie de données de validation

• 

  IHC staining of mouse kidney using 84801-4-RR (same clone as 84801-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84801-4-RR (CD9 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84801-4-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 84801-4-RR (same clone as 84801-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84801-4-RR (CD9 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84801-4-PBS in a different storage buffer formulation.

• 

  IF Staining of RAW 264.7 using 84801-4-RR (same clone as 84801-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using CD9 antibody (84801-4-RR, Clone: 242432C2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84801-4-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 84801-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84801-4-RR against Mouse CD9 were performed. The affinity constant is below 1 pM.

---

Informations sur le produit

84801-4-PBS cible CD9 dans les applications de IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD9 Protéine recombinante Eg1397
• Nom complet: CD9 antigen
• Masse moléculaire calculée: 25 kDa
• Numéro d’acquisition GenBank: NM_007657.3
• Symbole du gène: Cd9
• Identification du gène (NCBI): 12527
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD9, also known as Tspan-29, p24 or MIC3, is a member of the tetraspanin superfamily (PMID: 1879540). It is expressed on a large variety of hematopoietic and non-hematopoietic cells, such as stromal cells, megakaryocytes, platelets, B and T lymphocytes, dendritic cells, endothelial cells, mast cells, eosinophils, and basophils (PMID: 30356731). CD9 interacts with the integrin family and other membrane proteins, and is involved in cell adhesion, cell motility and tumor metastasis (PMID: 8478605; 21428940; 25805926). Expression of CD9 enhances membrane fusion between muscle cells and supports myotube maintenance (PMID:10459022). On oocytes, CD9 is hypothesized to play a role in the fertilization of mammals (PMID: 25536312).

{{ptg:RelatedPrimaryAntibodies}}