Anticorps Monoclonal anti-CD90

CD90 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2D7D11

N° de cat : 66766-1-PBS

Synonymes

CD90 / Thy1, THY1, 2D7D11, Thy 1 antigen, Thy 1 cell surface antigen

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Galerie de données de validation

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  WB analysis using 66766-1-Ig (same clone as 66766-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66766-1-Ig (CD90 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.

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  WB analysis of pig colon using 66766-1-Ig (same clone as 66766-1-PBS)

  pig colon tissue were subjected to SDS PAGE followed by western blot with 66766-1-Ig (CD90 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.

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  WB analysis of rabbit brain using 66766-1-Ig (same clone as 66766-1-PBS)

  rabbit brain tissue were subjected to SDS PAGE followed by western blot with 66766-1-Ig (CD90 antibody) at dilution of 1:9700 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.

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  WB analysis of U2OS using 66766-1-Ig (same clone as 66766-1-PBS)

  U2OS cells were subjected to SDS PAGE followed by western blot with 66766-1-Ig (CD90 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.

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  WB analysis of fetal human brain using 66766-1-Ig (same clone as 66766-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66766-1-Ig (CD90 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.

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  WB analysis of human kidney using 66766-1-Ig (same clone as 66766-1-PBS)

  human kidney tissue were subjected to SDS PAGE followed by western blot with 66766-1-Ig (CD90 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig brain using 66766-1-Ig (same clone as 66766-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66766-1-Ig (CD90 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig spleen using 66766-1-Ig (same clone as 66766-1-PBS)

  pig spleen tissue were subjected to SDS PAGE followed by western blot with 66766-1-Ig (CD90 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66766-1-Ig (same clone as 66766-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66766-1-Ig (CD90 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66766-1-Ig (same clone as 66766-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66766-1-Ig (CD90 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66766-1-Ig (same clone as 66766-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66766-1-Ig (CD90 antibody) at dilution of 1:3900 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66766-1-Ig (same clone as 66766-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66766-1-Ig (CD90 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66766-1-Ig (same clone as 66766-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66766-1-Ig (CD90 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66766-1-Ig (same clone as 66766-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66766-1-Ig (CD90 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 66766-1-Ig (same clone as 66766-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66766-1-Ig (CD90 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 66766-1-Ig (same clone as 66766-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66766-1-Ig (CD90 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66766-1-Ig (same clone as 66766-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66766-1-Ig (CD90 antibody) at dilution of 1:1000 (under 4x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 66766-1-Ig (same clone as 66766-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66766-1-Ig (CD90 antibody) at dilution of 1:3900 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 66766-1-Ig (same clone as 66766-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66766-1-Ig (CD90 antibody) at dilution of 1:3900 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66766-1-Ig (same clone as 66766-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66766-1-Ig (CD90 antibody) at dilution of 1:3900 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66766-1-Ig (same clone as 66766-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66766-1-Ig (CD90 antibody) at dilution of 1:3900 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 66766-1-Ig (same clone as 66766-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 66766-1-Ig (CD90 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 66766-1-Ig (same clone as 66766-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 66766-1-Ig (CD90 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66766-1-PBS cible CD90 dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CD90 Protéine recombinante Ag25603
• Nom complet: Thy-1 cell surface antigen
• Masse moléculaire calculée: 161 aa, 18 kDa
• Poids moléculaire observé: 28 kDa
• Numéro d’acquisition GenBank: BC065559
• Symbole du gène: CD90/Thy1
• Identification du gène (NCBI): 7070
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD90, also known as THY1, is a 25-35 kD protein that is expressed on 1-4% of human fetal liver cells, cord blood cells, and bone marrow cells. CD90 is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. Activation of Thy-1 has been reported to promote T cell activation. It also affects numerous nonimmunologic biological processes, including cellular adhesion, neurite outgrowth, tumor growth, migration, and cell death.

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