Anticorps Recombinant de lapin anti-CD90

CD90 Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241290B1

N° de cat : 84180-1-PBS

Synonymes

CD90 / Thy1, THY1, 241290B1, Thy 1 antigen, Thy 1 cell surface antigen

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Galerie de données de validation

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  WB analysis using 84180-1-RR (same clone as 84180-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84180-1-RR (CD90 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84180-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 84180-1-RR (same clone as 84180-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed SH-SY5Y cells using CD90 antibody (84180-1-RR, Clone: 241290B1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84180-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01072-1

  Cytometric bead array standard curve of MP01072-1, CD90 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84180-1-PBS. Detection antibody: 84180-2-PBS. Standard: RP02312. Range: 0.469-60 ng/mL.

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  Affinity and Kinetic Characterization of 84180-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84180-1-RR against Human CD90 were performed. The affinity constant is 0.383 nM.

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Informations sur le produit

84180-1-PBS cible CD90 dans les applications de WB, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: Thy-1 cell surface antigen
• Masse moléculaire calculée: 161 aa, 18 kDa
• Poids moléculaire observé: 25-35 kDa
• Numéro d’acquisition GenBank: BC065559
• Symbole du gène: CD90/Thy1
• Identification du gène (NCBI): 7070
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD90, also known as THY1, is a 25-35 kDa protein that is expressed on 1-4% of human fetal liver cells, cord blood cells, and bone marrow cells. CD90 is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. Activation of Thy-1 has been reported to promote T cell activation. It also affects numerous nonimmunologic biological processes, including cellular adhesion, neurite outgrowth, tumor growth, migration, and cell death.

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