Anticorps Polyclonal de lapin anti-CD90

CD90 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(10)

Avis (1)

N° de cat : 27178-1-AP

Synonymes

CD90 / Thy1, THY1, Thy 1 antigen, Thy 1 cell surface antigen, Thy 1 membrane glycoprotein

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Galerie de données de validation

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  WB analysis using 27178-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27178-1-AP (CD90 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 27178-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27178-1-AP (CD90 / Thy1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 27178-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27178-1-AP (CD90 / Thy1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 27178-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27178-1-AP (CD90 / Thy1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 27178-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27178-1-AP (CD90 / Thy1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 27178-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27178-1-AP (CD90 / Thy1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 27178-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27178-1-AP (CD90 / Thy1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 27178-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 27178-1-AP (CD90 / Thy1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 27178-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 27178-1-AP (CD90 / Thy1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de porc, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu cérébral de souris, tissu de cancer du poumon humain, tissu splénique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 10 publications below

Informations sur le produit

27178-1-AP cible CD90 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD90 Protéine recombinante Ag25603
• Nom complet: Thy-1 cell surface antigen
• Masse moléculaire calculée: 161 aa, 18 kDa
• Poids moléculaire observé: 26 kDa
• Numéro d’acquisition GenBank: BC065559
• Symbole du gène: CD90/Thy1
• Identification du gène (NCBI): 7070
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD90, also known as THY1, is a 25-35 kD protein that is expressed on 1-4% of human fetal liver cells, cord blood cells, and bone marrow cells. CD90 is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. Activation of Thy-1 has been reported to promote T cell activation. It also affects numerous nonimmunologic biological processes, including cellular adhesion, neurite outgrowth, tumor growth, migration, and cell death.

Publications

Species	Application	Title
rat	IF	Nat Commun; Injectable ECM-mimetic dynamic hydrogels abolish ferroptosis-induced post-discectomy herniation through delivering nucleus pulposus progenitor cell-derived exosomes; Authors - Wenkai Wang
mouse	IF	J Orthop Translat; Osteostaticytes: A novel osteoclast subset couples bone resorption and bone formation; Authors - Zhiyuan Wei
human	IF	Stem Cells; Nicotinamide riboside modulates HIF-1 signaling to maintain and enhance odontoblastic differentiation in human dental pulp stem cells; Authors - Peimeng Zhan
human	IF	Transl Oncol; A machine learning-based prognostic signature utilizing MSC proteomics for predicting bladder cancer prognosis and treatment response; Authors - Xinyu Zhang
rat	IF	Neurogastroenterol Motil; Construction and identification of an immortalized rat intestinal smooth muscle cell line.; Authors - Huiling Chen
rat	IF	ACS Nano; Micronano Titanium Accelerates Mesenchymal Stem Cells Aging through the Activation of Senescence-Associated Secretory Phenotype; Authors - Xuan Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Christelle (Verified Customer) (01-06-2025)

Immunofluorescent analysis of (PFA 4%) fixed human renal mesangial cells using CD90 antibody (27178-1-AP) at dilution of 1:50 and AF488-Conjugated Donkey Anti-Rabbit IgG (Life Technologies), Star Red membrane probe (Abberior, yellow) and DAPI (blue). 2 files uploaded : 1 aquisition without the membrane probe and the same aquisition with the membrane probe.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: Human Renal Mesangial Cells (HRMC, primary cells)

CD90 Antibody Immunofluorescence validation (1:50 dilution) in Human Renal Mesangial Cells (HRMC, primary cells) (Cat no:27178-1-AP)

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