Anticorps Recombinant de lapin anti-CD90

CD90 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

241388E2

N° de cat : 84206-3-RR

Synonymes

241388E2, Thy1, Thy-1, Thy-1 antigen, Thy-1 membrane glycoprotein

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Galerie de données de validation

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  WB analysis using 84206-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84206-3-RR (CD90/Thy1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of PC-12 using 84206-3-RR

  Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using CD90/Thy1 antibody (84206-3-RR, Clone: 241388E2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84206-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84206-3-RR against Rat CD90/Thy1 were performed. The affinity constant is 27.1 pM.

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Applications testées

• Résultats positifs en WB: cellules PC-12, tissu cérébral de rat
• Résultats positifs en IF/ICC: cellules PC-12,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84206-3-RR cible CD90 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons rat

• Réactivité: rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD90 Protéine recombinante Eg1463
• Nom complet: Thy-1 cell surface antigen
• Masse moléculaire calculée: 18 kDa
• Poids moléculaire observé: 25 kDa
• Numéro d’acquisition GenBank: NM_012673.2
• Symbole du gène: Thy1
• Identification du gène (NCBI): 24832
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD90, also known as THY1, is a 25-35 kD protein that is expressed on 1-4% of human fetal liver cells, cord blood cells, and bone marrow cells. CD90 is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. Activation of Thy-1 has been reported to promote T cell activation. It also affects numerous nonimmunologic biological processes, including cellular adhesion, neurite outgrowth, tumor growth, migration, and cell death.

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