Anticorps Monoclonal anti-CD97

CD97 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3F10C2

N° de cat : 66972-1-PBS

Synonymes

CD97, CD97 antigen, CD97 molecule, Leukocyte antigen CD97, TM7LN1

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Galerie de données de validation

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  WB analysis of K-562 using 66972-1-Ig (same clone as 66972-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 66972-1-Ig (CD97 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66972-1-PBS in a different storage buffer formulation.

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  WB analysis of HL-60 using 66972-1-Ig (same clone as 66972-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 66972-1-Ig (CD97 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66972-1-PBS in a different storage buffer formulation.

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  WB analysis of DC2.4 using 66972-1-Ig (same clone as 66972-1-PBS)

  DC2.4 cells were subjected to SDS PAGE followed by western blot with 66972-1-Ig (CD97 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66972-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66972-1-Ig (same clone as 66972-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66972-1-Ig (CD97 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66972-1-PBS in a different storage buffer formulation.

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  IF Staining of Jurkat using 66972-1-Ig (same clone as 66972-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD97 antibody (66972-1-Ig, Clone: 3F10C2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66972-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66972-1-PBS cible CD97 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CD97 Protéine recombinante Ag27991
• Nom complet: CD97 molecule
• Masse moléculaire calculée: 722 aa, 79 kDa
• Poids moléculaire observé: 75 kDa
• Numéro d’acquisition GenBank: BC026690
• Symbole du gène: CD97
• Identification du gène (NCBI): 976
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD97 is a member of the seven-trans-membrane subfamily of the class B G protein-coupled receptor (GPCR) group of the epidermal growth factor (EGF) and is present on the surface of lymphocytes, monocytes, macrophages, dendritic cells, granulocytes, and smooth muscle, being a dimeric glycoprotein with a 75-90 kDa intracellular domain and a 28 kDa extracellular domain (PMID: 23969601). It plays a vital role in cell adhesion, migration, and cell connection regulation (PMID: 36464675). CD97 is expressed on T-cells, but rarely on B cells. CD97 might play key roles in cellular adhesion, through connections with other proteins of the cell surface and of the extracellular matrix (PMID: 32781778).

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