Anticorps Recombinant de lapin anti-CD98/SLC3A2

CD98/SLC3A2 Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241247F12

N° de cat : 84040-7-PBS

Synonymes

4F2 cell-surface antigen heavy chain, 4F2hc, Amino acid transporter heavy chain SLC3A2, CD98, Mdu1

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Galerie de données de validation

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  WB analysis of mouse kidney using 84040-7-RR (same clone as 84040-7-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 84040-7-RR (CD98 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84040-7-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 84040-7-RR (same clone as 84040-7-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 84040-7-RR (CD98 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84040-7-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 84040-7-RR (same clone as 84040-7-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84040-7-RR (CD98 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84040-7-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 84040-7-RR (same clone as 84040-7-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84040-7-RR (CD98 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84040-7-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 84040-7-RR (same clone as 84040-7-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84040-7-RR (CD98 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84040-7-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 84040-7-RR (same clone as 84040-7-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84040-7-RR (CD98 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84040-7-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 84040-7-RR

  Biolayer interferometry (BLl) kinetic assays of 84040-7-RR against Mouse CD98/SLC3A2 were performed. The affinity constant is 1.36 nM.

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Informations sur le produit

84040-7-PBS cible CD98/SLC3A2 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD98/SLC3A2 Protéine recombinante Eg1374
• Nom complet: solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2
• Masse moléculaire calculée: 58 kDa
• Numéro d’acquisition GenBank: NM_008577.4
• Symbole du gène: CD98/SLC3A2
• Identification du gène (NCBI): 17254
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD98 is a cell-surface heterodimer consisting of a heavy chain (CD98hc) and a light chain. CD98hc also interacts with certain integrin b-subunits to regulate cell migration, survival, proliferation, and adhesion/polarity. CD98hc is overexpressed on the cell surface of many cancers and increased CD98hc expression is associated with the development and progression of tumors.(PMID:25084765). This protein has 4 isoforms with the molecular mass of 58-71 kDa and can be detected 85-94 kDa due to glycosylation. The glycosylated CD98hc can links to a non-glycosylated light chain (~40 kDa) via a disulfide bond to form a heterodimeric CD98 antigen with molecular mass of 120-130 kDa (PMID: 14770309).

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