Anticorps Recombinant de lapin anti-CD98/SLC3A2

CD98/SLC3A2 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

241755A11

N° de cat : 84387-6-RR

Synonymes

CD98, SLC3A2, 241755A11, 4F2 cell-surface antigen heavy chain, Amino acid transporter heavy chain SLC3A2

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Galerie de données de validation

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  WB analysis using 84387-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84387-6-RR (CD98 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 84387-6-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CD98 antibody (84387-6-RR, Clone: 241755A11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of A431 using 84387-6-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CD98/SLC3A2 antibody (84387-6-RR, Clone: 241755A11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules HeLa,
• Résultats positifs en IF/ICC: cellules HepG2, cellules A431

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84387-6-RR cible CD98/SLC3A2 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD98/SLC3A2 Protéine recombinante Eg1948
• Nom complet: solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2
• Masse moléculaire calculée: 68 kDa
• Poids moléculaire observé: 68-125 kDa
• Numéro d’acquisition GenBank: NM_002394.5
• Symbole du gène: CD98
• Identification du gène (NCBI): 6520
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD98 is a 125-kDa type II membrane glycoprotein heterodimer composed of a 40-kDa non-glycosylated light chain (amino-acid transporter) and an 85-kDa glycosylated heavy chain (CD98hc) (PMID: 11507094). CD98hc also interacts with certain integrin b-subunits to regulate cell migration, survival, proliferation, and adhesion/polarity. CD98hc is overexpressed on the cell surface of many cancers and increased CD98hc expression is associated with the development and progression of tumors.(PMID:25084765). Molecular orientation studies have demonstrated that the C-terminal tail of human CD98 (hCD98), which contains a PDZ-binding domain, is extracellular (PMID: 18625289).

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