Anticorps Monoclonal anti-CD99

CD99 Monoclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

5B6A4

N° de cat : 60354-1-PBS

Synonymes

12E7, CD99, CD99 antigen, CD99 molecule, E2 antigen, MIC2, MIC2X, MIC2Y, Protein MIC2, T cell surface glycoprotein E2

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Galerie de données de validation

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  WB analysis of human spleen using 60354-1-Ig (same clone as 60354-1-PBS)

  human spleen tissue were subjected to SDS PAGE followed by western blot with 60354-1-Ig (CD99 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60354-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 60354-1-Ig (same clone as 60354-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 60354-1-Ig (CD99 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60354-1-PBS in a different storage buffer formulation.

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Informations sur le produit

60354-1-PBS cible CD99 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CD99 Protéine recombinante Ag19474
• Nom complet: CD99 molecule
• Masse moléculaire calculée: 19 kDa, 16 kDa, 17 kDa
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: BC021620
• Symbole du gène: CD99
• Identification du gène (NCBI): 4267
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD99, also known as MIC2, is a heavily O-glycosylated transmembrane protein involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. CD99 is broadly distributed on many cell types, with particularly strong expression on human cortical thymocytes, Ewing’s sarcoma cells and peripheral primitive neuroectodermal tumors (PMID: 9794396; 16984917). In normal cells, CD99 has been functionally implicated in cell adhesion, migration, apoptosis, differentiation, activation, and proliferation of lymphocytes and monocyte extravasation and transport of several transmembrane proteins (PMID: 16984917). CD99 displays two surface isoforms generated by alternative splicing: a long 32 kDa and a short 28 kDa form (PMID: 12368226).

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