Anticorps Recombinant de lapin anti-CD99

CD99 Recombinant Antibody for FC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241737H1

N° de cat : 98174-1-PBS

Synonymes

12E7, 241737H1, CD99 antigen, CD99 molecule, E2 antigen

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Galerie de données de validation

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  FC experiment of human PBMCs using 98174-1-RR (same clone as 98174-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD99 Rabbit Recombinant Antibody (98174-1-RR, Clone:241737H1) or 0.25 ug Isotype Control, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98174-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 98174-1-RR (same clone as 98174-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD99 Rabbit Recombinant Antibody (98174-1-RR, Clone:241737H1) (red) or 0.25 ug Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98174-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98174-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98174-1-RR against Human CD99 were performed. The affinity constant is 3.61 nM.

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Informations sur le produit

98174-1-PBS cible CD99 dans les applications de FC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: CD99 molecule
• Masse moléculaire calculée: 19 kDa, 16 kDa, 17 kDa
• Numéro d’acquisition GenBank: BC021620
• Symbole du gène: CD99
• Identification du gène (NCBI): 4267
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C.

Informations générales

CD99, also known as MIC2, is a heavily O-glycosylated transmembrane protein involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. CD99 is broadly distributed on many cell types, with particularly strong expression on human cortical thymocytes, Ewing's sarcoma cells and peripheral primitive neuroectodermal tumors (PMID: 9794396; 16984917). In normal cells, CD99 has been functionally implicated in cell adhesion, migration, apoptosis, differentiation, activation, and proliferation of lymphocytes and monocyte extravasation and transport of several transmembrane proteins (PMID: 16984917).

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