Anticorps Recombinant de lapin anti-CD99

CD99 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

CloneNo.

241737H1

N° de cat : 98174-1-RR

Synonymes

MIC2, E2 antigen, CD99 molecule, CD99 antigen, 241737H1

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Galerie de données de validation

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  FC experiment of human PBMCs using 98174-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD99 Rabbit Recombinant Antibody (98174-1-RR, Clone:241737H1) or 0.25 ug Isotype Control, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

• 

  FC experiment of human PBMCs using 98174-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD99 Rabbit Recombinant Antibody (98174-1-RR, Clone:241737H1) (red) or 0.25 ug Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98174-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98174-1-RR against Human CD99 were performed. The affinity constant is 3.61 nM.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98174-1-RR cible CD99 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: CD99 molecule
• Masse moléculaire calculée: 19 kDa, 16 kDa, 17 kDa
• Numéro d’acquisition GenBank: BC021620
• Symbole du gène: CD99
• Identification du gène (NCBI): 4267
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

CD99, also known as MIC2, is a heavily O-glycosylated transmembrane protein involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. CD99 is broadly distributed on many cell types, with particularly strong expression on human cortical thymocytes, Ewing's sarcoma cells and peripheral primitive neuroectodermal tumors (PMID: 9794396; 16984917). In normal cells, CD99 has been functionally implicated in cell adhesion, migration, apoptosis, differentiation, activation, and proliferation of lymphocytes and monocyte extravasation and transport of several transmembrane proteins (PMID: 16984917).

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