Anticorps Recombinant de lapin anti-CDC20

• Phare
• Validé par KD/KO

CDC20 Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241925E12

N° de cat : 84531-5-PBS

Synonymes

241925E12, CDC20A, p55CDC

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Galerie de données de validation

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  WB analysis using 84531-5-RR (same clone as 84531-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84531-5-RR (CDC20 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84531-5-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 84531-5-RR (same clone as 84531-5-PBS)

  WB result of CDC20 antibody (84531-5-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CDC20 transfected HeLa cells. This data was developed using the same antibody clone with 84531-5-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 84531-5-RR (same clone as 84531-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84531-5-RR (CDC20 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84531-5-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 84531-5-RR (same clone as 84531-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84531-5-RR (CDC20 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84531-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84531-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84531-5-RR against Human CDC20 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

84531-5-PBS cible CDC20 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CDC20 Protéine recombinante Ag0295
• Nom complet: cell division cycle 20 homolog (S. cerevisiae)
• Masse moléculaire calculée: 55 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC001088
• Symbole du gène: CDC20
• Identification du gène (NCBI): 991
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Cdc20 (Cell-division cycle protein 20 homologue) is also named as p55CDC and belongs to the WD repeat CDC20/Fizzy family. Cdc20 has important functions in chromosome segregation and mitotic exit. Cdc20 is the target of the spindle assembly checkpoint (SAC) and a key cofactor of the anaphase-promoting complex or cyclosome (APC/CCDC20) E3 ubiquitin ligase, thus regulating APC/C ubiquitin activity on specific substrates for their subsequent degradation by the proteasome (PMID: 28202332). The 55-kDa band corresponded to the size of Cdc20 (PMID: 22566641).

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