Anticorps Polyclonal de lapin anti-CDC25A

• Phare
• Validé par KD/KO

CDC25A Polyclonal Antibody for WB, IHC, IF-P, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF-P, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 55031-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of HepG2 using 55031-1-AP (same clone as 55031-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55031-1-AP (CDC25A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55031-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 55031-1-AP (same clone as 55031-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 55031-1-AP (CDC25A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55031-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 55031-1-AP (same clone as 55031-1-PBS)

  IP result of anti-CDC25A (IP:55031-1-AP, 4ug; Detection:55031-1-AP 1:300) with HeLa cells lysate 4000ug. This data was developed using the same antibody clone with 55031-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 55031-1-AP (same clone as 55031-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55031-1-AP (CDC25A Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55031-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 55031-1-AP (same clone as 55031-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55031-1-AP (CDC25A Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55031-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 55031-1-AP (same clone as 55031-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55031-1-AP (CDC25A Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55031-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 55031-1-AP (same clone as 55031-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55031-1-AP (CDC25A Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55031-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 55031-1-AP (same clone as 55031-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 55031-1-AP (CDC25A Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55031-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 55031-1-AP (same clone as 55031-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 55031-1-AP (CDC25A Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55031-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human breast cancer using 55031-1-AP (same clone as 55031-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 55031-1-AP (CDC25A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 55031-1-PBS in a different storage buffer formulation.

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Informations sur le produit

55031-1-PBS cible CDC25A dans les applications de WB, IHC, IF-P, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: cell division cycle 25 homolog A (S. pombe)
• Masse moléculaire calculée: 59 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: NM_001789
• Symbole du gène: CDC25A
• Identification du gène (NCBI): 993
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CDC25A belongs to the MPI phosphatase family. It is a tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. CDC25A directly dephosphorylates CDC2 and stimulates its kinase activity. It also dephosphorylates CDK2 in complex with cyclin E, in vitro. CDC25A overexpression in human cancers might contribute to tumorigenesis. This protein can be phosphorylated to reveal the molecular weight from 55 kDa to 70 kDa(PMID:21376736; 8156993).The antibody is specific to CDC25A.

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