Anticorps Recombinant de lapin anti-CDC25A

CDC25A Recombinant Antibody for IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

240813F3

N° de cat : 84145-2-PBS

Synonymes

M-phase inducer phosphatase 1, EC:3.1.3.48, CDC25A2, CDC25, 240813F3

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Galerie de données de validation

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  IHC staining of human stomach cancer using 84145-2-RR (same clone as 84145-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84145-2-RR (CDC25A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84145-2-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 84145-2-RR (same clone as 84145-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84145-2-RR (CDC25A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84145-2-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 84145-2-RR (same clone as 84145-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CDC25A antibody (84145-2-RR, Clone: 240813F3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84145-2-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 84145-2-RR (same clone as 84145-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CDC25A antibody (84145-2-RR, Clone: 240813F3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84145-2-PBS in a different storage buffer formulation.

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Informations sur le produit

84145-2-PBS cible CDC25A dans les applications de IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: cell division cycle 25 homolog A (S. pombe)
• Masse moléculaire calculée: 59 kDa
• Numéro d’acquisition GenBank: NM_001789
• Symbole du gène: CDC25A
• Identification du gène (NCBI): 993
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CDC25A belongs to the MPI phosphatase family. It is a tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. CDC25A directly dephosphorylates CDC2 and stimulates its kinase activity. It also dephosphorylates CDK2 in complex with cyclin E, in vitro. CDC25A overexpression in human cancers might contribute to tumorigenesis. This protein can be phosphorylated to reveal the molecular weight from 55 kDa to 70 kDa(PMID:21376736; 8156993).The antibody is specific to CDC25A.

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