Anticorps Monoclonal anti-CDC25B

CDC25B Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1H3A4

N° de cat : 67145-1-PBS

Synonymes

M phase inducer phosphatase 2, EC:3.1.3.48, Cell division cycle 25 homolog B, CDC25HU2, Cdc 25B

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Galerie de données de validation

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  WB analysis of Sp2/0 using 67145-1-Ig (same clone as 67145-1-PBS)

  Sp2/0 cells were subjected to SDS PAGE followed by western blot with 67145-1-Ig (CDC25B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67145-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 67145-1-Ig (same clone as 67145-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67145-1-Ig (CDC25B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67145-1-PBS in a different storage buffer formulation.

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  WB analysis of U-937 using 67145-1-Ig (same clone as 67145-1-PBS)

  U-937 cells were subjected to SDS PAGE followed by western blot with 67145-1-Ig (CDC25B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67145-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 67145-1-Ig (same clone as 67145-1-PBS)

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 67145-1-Ig (CDC25B antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67145-1-PBS in a different storage buffer formulation.

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  IF Staining of Jurkat using 67145-1-Ig (same clone as 67145-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CDC25B antibody (67145-1-Ig, Clone: 1H3A4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67145-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67145-1-PBS cible CDC25B dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CDC25B Protéine recombinante Ag27193
• Nom complet: cell division cycle 25 homolog B (S. pombe)
• Masse moléculaire calculée: 65 kDa
• Poids moléculaire observé: 69 kDa
• Numéro d’acquisition GenBank: BC006395
• Symbole du gène: CDC25B
• Identification du gène (NCBI): 994
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CDC25B, also named as CDC25HU2, belongs to the MPI phosphatase family. It is a tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. CDC25B directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.

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