Anticorps Monoclonal anti-CDC27; APC3

CDC27; APC3 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2F10G2

N° de cat : 67239-1-PBS

Synonymes

CDC27, CDC27 homolog, APC3, APC 3, Anaphase-promoting complex subunit 3

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Galerie de données de validation

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  WB analysis of Jurkat using 67239-1-Ig (same clone as 67239-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67239-1-Ig (CDC27; APC3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67239-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 67239-1-Ig (same clone as 67239-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67239-1-Ig (CDC27; APC3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67239-1-PBS in a different storage buffer formulation.

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  WB analysis of 4T1 using 67239-1-Ig (same clone as 67239-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 67239-1-Ig (CDC27; APC3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67239-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 67239-1-Ig (same clone as 67239-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67239-1-Ig (CDC27; APC3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67239-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 67239-1-Ig (same clone as 67239-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67239-1-Ig (CDC27; APC3 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67239-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 67239-1-Ig (same clone as 67239-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67239-1-Ig (CDC27; APC3 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67239-1-PBS in a different storage buffer formulation.

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  IF Staining of human lung cancer using 67239-1-Ig (same clone as 67239-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CDC27; APC3 antibody (67239-1-Ig, Clone: 2F10G2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67239-1-PBS in a different storage buffer formulation.

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  IF Staining of human lung cancer using 67239-1-Ig (same clone as 67239-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CDC27; APC3 antibody (67239-1-Ig, Clone: 2F10G2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67239-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 67239-1-Ig (same clone as 67239-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CDC27; APC3 antibody (67239-1-Ig, Clone: 2F10G2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67239-1-PBS in a different storage buffer formulation.

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  FC experiment of K-562 using 67239-1-Ig (same clone as 67239-1-PBS)

  1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human CDC27; APC3 (67239-1-Ig, Clone:2F10G2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67239-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67239-1-PBS cible CDC27; APC3 dans les applications de WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CDC27; APC3 Protéine recombinante Ag25588
• Nom complet: cell division cycle 27 homolog (S. cerevisiae)
• Masse moléculaire calculée: 92 kDa
• Poids moléculaire observé: 90-100 kDa
• Numéro d’acquisition GenBank: BC011656
• Symbole du gène: CDC27
• Identification du gène (NCBI): 996
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CDC27/APC3 is a component of the anaphase-promoting complex (APC/cyclosome), which is composed of eight subunits and highly conserved in eukaryotic cells. The APC/cyclosome complex acts as a cell cycle-regulated E3 ubiquitin ligase which mediates ubiquitination and subsequent degradation of target proteins, and lead to the progression control through mitosis and the G1 phase of the cell cycle.

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