Anticorps Polyclonal de lapin anti-CDK8/19

CDK8/19 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 13761-1-AP

Synonymes

CDC2L6, CDC2 related protein kinase 6, CDC2-related protein kinase 6, CDK11, CDK19

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Galerie de données de validation

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  WB analysis of Y79 using 13761-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 13761-1-AP (CDK8/19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 13761-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13761-1-AP (CDC2L6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 13761-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 13761-1-AP (CDK8/19 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 13761-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13761-1-AP (CDK8/19 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 13761-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13761-1-AP (CDK8/19 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Y79, tissu cérébral de souris
• Résultats positifs en IHC: tissu testiculaire de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:300-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

13761-1-AP cible CDK8/19 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CDK8/19 Protéine recombinante Ag4731
• Nom complet: cell division cycle 2-like 6 (CDK8-like)
• Masse moléculaire calculée: 502 aa, 57 kDa
• Poids moléculaire observé: 50-57 kDa
• Numéro d’acquisition GenBank: BC037289
• Symbole du gène: CDK19
• Identification du gène (NCBI): 23097
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The CDK8 homolog, CDK19 (Cdk11, CDC2L6) was discovered by Hidden Markov model (HMM) profiling of a eukaryotic protein kinase domain, and found to have approximately 91% sequence homology to Cdk8. The two proteins share a particularly high degree of sequence conservation in their kinase and cyclin binding domain (PMID: 30693281, 26985305). CDK8 and CDK19, collectively termed 'Mediator Kinase,' are incorporated, in a mutually exclusive manner, as part of a 4-protein complex called the Mediator kinase module. CDK8/19 inhibition suppressed the induction of NFκB-driven transcription by p21 (PMID: 28855340).

Publications

Species	Application	Title
human	WB,IHC	Cell Mol Life Sci; Hotair promotes the migration and proliferation in ovarian cancer by miR-222-3p/CDK19 axis.; Authors - Lili Fan

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