Anticorps Recombinant de lapin anti-CDC40

CDC40 Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242034D1

N° de cat : 84679-4-PBS

Synonymes

242034D1, Cell division cycle 40 homolog, EH binding protein 3, EHB3, hPRP17

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Galerie de données de validation

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  WB analysis using 84679-4-RR (same clone as 84679-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84679-4-RR (CDC40 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84679-4-PBS in a different storage buffer formulation.

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  IF Staining of K-562 using 84679-4-RR (same clone as 84679-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using CDC40 antibody (84679-4-RR, Clone: 242034D1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84679-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84679-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84679-4-RR against Human CDC40 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

84679-4-PBS cible CDC40 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CDC40 Protéine recombinante Ag36151
• Nom complet: cell division cycle 40 homolog (S. cerevisiae)
• Poids moléculaire observé: 65-67 kDa
• Numéro d’acquisition GenBank: BC126114
• Symbole du gène: CDC40
• Identification du gène (NCBI): 51362
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CDC40 (cell division cycle 40), also known as EHB3. It is expected to be located in the nucleus, which is ubiquitinated in bone marrow and lymph node. The protein is required for pre-mRNA splicing as component of the activated spliceosome, and it plays an important role in embryonic brain development; this function does not require proline isomerization (PMID: 33220177; 33220177). The molecular weight of CDC40 is 65 kDa.

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