Anticorps Recombinant de lapin anti-CDC40

CDC40 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

242034D1

N° de cat : 84679-4-RR

Synonymes

242034D1, Cell division cycle 40 homolog, EH binding protein 3, EHB3, hPRP17

---

Galerie de données de validation

• 

  WB analysis using 84679-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84679-4-RR (CDC40 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of K-562 using 84679-4-RR

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using CDC40 antibody (84679-4-RR, Clone: 242034D1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  Affinity and Kinetic Characterization of 84679-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84679-4-RR against Human CDC40 were performed. The affinity constant is below 1 pM.

---

Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules HeLa, cellules HUVEC, cellules Jurkat, cellules K-562, cellules THP-1
• Résultats positifs en IF/ICC: cellules K-562,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84679-4-RR cible CDC40 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CDC40 Protéine recombinante Ag36151
• Nom complet: cell division cycle 40 homolog (S. cerevisiae)
• Poids moléculaire observé: 65-67 kDa
• Numéro d’acquisition GenBank: BC126114
• Symbole du gène: CDC40
• Identification du gène (NCBI): 51362
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CDC40 (cell division cycle 40), also known as EHB3. It is expected to be located in the nucleus, which is ubiquitinated in bone marrow and lymph node. The protein is required for pre-mRNA splicing as component of the activated spliceosome, and it plays an important role in embryonic brain development; this function does not require proline isomerization (PMID: 33220177; 33220177). The molecular weight of CDC40 is 65 kDa.

{{ptg:RelatedPrimaryAntibodies}}