Anticorps Polyclonal de lapin anti-CDC42EP4

CDC42EP4 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 19262-1-AP

Synonymes

CEP4, Cdc42 effector protein 4, BORG4, Binder of Rho GTPases 4

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Galerie de données de validation

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  WB analysis of K-562 using 19262-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 19262-1-AP (CDC42EP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 19262-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 19262-1-AP (CDC42EP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 19262-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 19262-1-AP (CDC42EP4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human intrahepatic cholangiocarcinoma using 19262-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 19262-1-AP (CDC42EP4 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 19262-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 19262-1-AP (CDC42EP4 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules A549, tissu pulmonaire de souris
• Résultats positifs en IHC: human intrahepatic cholangiocarcinoma tissue, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

19262-1-AP cible CDC42EP4 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CDC42EP4 Protéine recombinante Ag13594
• Nom complet: CDC42 effector protein (Rho GTPase binding) 4
• Masse moléculaire calculée: 38 kDa
• Poids moléculaire observé: 43-45 kDa
• Numéro d’acquisition GenBank: BC010451
• Symbole du gène: CDC42EP4
• Identification du gène (NCBI): 23580
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CDC42EP4, or Cell division cycle 42 effector protein 4, is a member of the CDC42-binding protein family. This protein interacts with Rho family GTPases and plays a role in regulating the organization of the actin cytoskeleton. It has been shown to bind both CDC42 and TC10 GTPases in a GTP-dependent manner. Overexpression of CDC42EP4 in fibroblasts can induce pseudopodia formation, suggesting its involvement in actin filament assembly and cell shape control. CDC42EP4 is also implicated in cancer progression, particularly in prostate cancer. Furthermore, CDC42EP4 has been implicated in the regulation of glutamate transporters, which are crucial for maintaining glutamate homeostasis in the nervous system. It has been suggested that CDC42EP4 may interact with septins, a family of polymerizing GTPases that constitute the membrane skeleton, and this interaction could potentially influence the localization and activity of glutamate transporters.

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